Q exactive hfx. Q-Exactive Plus Thermo Scientific Web Materials.

• Q exactive hfx The total ion chromatograms (TICs) of the QC, CS, The Q Exactive HF-X mass spectrometer uses a high-capacity transfer tube to maximize the loading of ions, a motorized ion funnel to contain and transfer a wider mass range of ions, and a high-field Thermo Scientific™ Orbitrap™ mass analyzer. MS/MS spectra can have 15 ms longer IT on the Q Exactive HF-X and still have the same acquisition time compared to the Q Exactive HF. 5 1357. However, the tuber quality in shaded environments is unknown, which limits the scientific cultivation of P. Together, these components enable rapid peptide identification and analysis, label-free and TMT The Q Exactive HF mass spectrometer supports multiple approaches to quantitation including selected ion monitoring (SIM), parallel-reaction monitoring (PRM), and data-independent acquisition (DIA). Shimadzu LCMS-8050. The superior quality of Q Exactive MS/MS data enables identification and quantitation of more compounds with greater confidence. WATERS SQD. Clear All. Huhmer 1 Thermo Fisher Scientific, San Jose, CA 95134, USA 1; Thermo Fisher Scientific, Hanna-Kunath-Str. TSQ Quantum Access MAX Triple Quadrupole Mass Spectrometer Support › TSQ Endura Triple Quadrupole Mass Liquid chromatography was performed using a UHPLC system (Vanquish, Thermo Fisher Scientific) with a UPLC BEH Amide column (2. This instrument is a High Mass Resolution quadrupole Orbitrap mass spectrometer that contains a high capacity transfer tube and electrodynamic ion funnel to maximize ion signal over a broad mass range. 1 mm × 100 mm, 1. The Q Exactive also employs a 90° bent ion path from the source toward the mass analyzer in common with the Exactive and in contrast to the LTQ Orbitrap instruments. The Thermo Scientific ™  Q Exactive ™  HF-× Hybrid Quadrupole-Orbitrap ™  Mass Spectrometer rises to the occasion and sets new standards in sensitivity, performance and productivity The Q Exactive HF-× Mass Spectrometer uses a high-capacity transfer tube for maximum ion loading, an electrodynamic ion funnel that accomodates and transmits ions over A total of 2084 ESI + and 1294 ESI − compounds were identified in the primary (MS1) and secondary (MS2) metabolite profiles. 7 μm) coupled to Q Performance Evaluation of the Q Exactive HFX for Shotgun Proteomics doi 10. About This Guide Q Exactive HF-X Hybrid Quadrupole-Orbitrap Mass Spectrometer – Troubleshooting. We Exactive Orbitrap tandem mass spectrometry (UPLC-Q-Exactive Orbitrap/MS) detection for the discrimination of components from CSF was set up for the first time in this work. Hamburger Menu Button. The defining difference of the Q Exactive compared with the Which Q Exactive Orbitrap Mass Spectrometer is best for my lab? Our hybrid quadrupole-Orbitrap mass spectrometry systems vary in terms of mass resolution, scan speed and analysis modes. About This Guide Q Exactive HFX with Acqity H-class: TSQ Vantage with Classic Acquity: LTQ with Surveyor LC system: Our instruments maintained by experienced personel at regular intervals and the instrument manufacturer services most of our platforms under annual contracts. Improved productivity with Q Exactive HF MS The Q Exactive HF MS system completes 1 full MS scan 31. jproteome. Lets go to the Q Exactive Family Cycle Time Calculator! (Which you can download here). A Lets go to the Q Exactive Family Cycle Time Calculator! (Which you can download here). Q Exactive HF-X. This guide is intended for all personnel The novel Q Exactive HF-X MS acquires MS/MS spectra at twice the speed compared to the Q Exactive HF MS with a maximum acquisition frequency of 40Hz. The defining difference of the Q Exactive compared with the Exactive is the presence of a mass selective quadrupole analyzer between the ion source and the C-trap . , 2007), hierarchical cluster analysis (HCA) (Kolde and Kolde, 2015), orthogonal partial least squares-discrimination analysis (OPLS-DA) (Bylesjo et al. Superior ion Q Exactive HF-X Hybrid Quadrupole Orbitrap Mass Spectrometer Support - Troubleshooting › Find valuable information. The Thermo Scientific™ Q Exactive™ UHMR (Ultra-High Mass Range) Q Exactive HF-X Hybrid Quadrupole Orbitrap Mass Spectrometer Support - Getting Started Q Exactive HF-X Hybrid Quadrupole Orbitrap Mass Spectrometer Support - Troubleshooting Q c only Q Exactive HF-X : In the simplest case, set the max IT to times just below these values – parallel acquisition. The group used 1 μg of HeLa tryptic digest as a standard sample, loaded on column, and found that the Q Exactive HF-X MS delivered a 50% gain in the number of peptides identified if the The Q Exactive HF-X Mass Spectrometer uses a high-capacity transfer tube for maximum ion loading, an electrodynamic ion funnel that accomodates and transmits ions over a broader mass range, and a high-field Thermo PDF | On May 1, 2024, Michal Greguš and others published Improved Data Acquisition Settings on Q Exactive HF-X and Fusion Lumos Tribrid Orbitrap-Based Mass Spectrometers for Proteomic Analysis of The Thermo Scientific™ Q Exactive™ Plus, Q Exactive HF and Q Exactive HF-X Hybrid Quadrupole-Orbitrap™ mass spectrometers add superior denatured and native MS intact analysis and subunit top/middle-down analysis capabilities to capabilities, the Q Exactive mass spectro-meter is an outstanding detector for fast chromatography separation techniques. Vintage. Thermo Scientific ™ Acclaim RSLC 120, C18, 2. •The toolbar provides symbol shortcuts for frequently used Q Exactive HF-X Hybrid Quadrupole Orbitrap Mass Spectrometer Support - Getting Started Q Exactive HF-X Hybrid Quadrupole Orbitrap Mass Spectrometer Support - Troubleshooting Q Exactive HF Hybrid Quadrupole Orbitrap Mass Spectrometer Support › Q Exactive Plus Hybrid Quadrupole Orbitrap Mass Spectrometer Support › The Q Exactive HF-X MS takes identifi cation and quantitation to an unmatched level, making it possible to identify, quantify, and confi rm in a single analysis with a single instrument. 7 μm), coupled to a Q . Setting up DIA methods on Orbitrap analyzers requires a thorough overview of the actions the Orbitrap mass spectrometers carry out. The combination of the quaternary binary unit and the 19 Tesla field homogeneity ensures low-mass striping and leading performance for even the Application Note: TMT Workflow on the Q Exactive Series - Instrument Parameter Optimization and Data Analysis in Proteome Discoverer 2. The instrument raises the standards in state-of-art proteomics with its sensitivity, performance, and For each fraction, 1ug peptide were separated and analyzed with a nano­UPLC (EASY­nLC1200) coupled to a Q Exactive HFX Orbitrap instrument (Thermo Fisher Scientific) with a Systematic optimization of the DIA method on a Q Exactive HF instrument using a HeLa lysate and 2 h gradients. Thermo Scientific Q Exactive Mass Spectrometer. Se connecter. Full MS Figure The Thermo Scientific™ Q Exactive™ Hybrid Quadrupole-Orbitrap™ Mass Spectrometer introduced the revolutionary concept of Quanfirmation. Here we benchmarked the performance of the latest MS instrument in the benchtop Orbitrap series, the Q Exactive HF-X, against its predecessor for proteomics applications. Performance Characteristics Resolution 240,000 at m/z 200 Mass range 50 to 6,000 m/z Scan rate* Up to 40 Hz at resolution setting of 7,500 at The Q-Exactive HF is a hybrid quadrupole-orbitrap mass spectrometer, which combines the ultra-high field orbitrap mass analyser with a quadrupole mass analyser. 超分辨率液质联用仪（Q Exactive HF-X） Q Exactive HF-X 质谱仪具有低至fg级的灵敏度、高达24万的分辨率和不少于18 Hz的扫描速度，以及抗污染的分段式四级杆，为深度分析、定量准确性和重现性设定了新的标准，为生命科学研究和生物制药应用的挑战性工作提供卓越的数据质量，可用于综合蛋白质组分析 Q-Exactive HFx Scan speed up to 40 Hz with ultra-high-field Thermo Scientific™ Orbitrap™ mass analyzer technology • Resolution of up to 240,000 (FWHM) at m/z 200 Q-Exactive Plus Thermo Scientific Web Materials. The conditions of the Perform the highest-quality native, intact mass, and top-down analysis in structural biology and biopharma research. After standardization of the qualitative and quantitative results of all metabolites using the data z-score in the R package “pheatmap” (version 1. ternata. However, this might Benefit from record-setting performance. The Q Exactive HF-X MS provides best-in-class performance in peptides, proteins, and small molecule applications. 7 μm) coupled to Q Exactive HFX mass spectrometer (Orbitrap MS, Thermo). In addition to common attributes of an Orbitrap Q Exactive Resolution at m/z 200 "Free" injection time [ms] Approx. Orbitrap mass analyzer-based mass spectrometers have been well known for high resolution and accurate mass detection capabilities. It should be mentioned acquisition methods I, II, and III detected 22, 24, and 23 pesticides spiked at 500 μg/kg in sample by RTP, respectively, and 14, 14, and 16 pesticides by RTFI, respectively, out of 31 Q Exactive HF-X Hybrid Quadrupole-Orbitrap Mass Spectrometer Support. The peptide samples were taken up in 20 μl of buffer A c only Q Exactive HF-X : In the simplest case, set the max IT to times just below these values – parallel acquisition. 16 maxIT . cicadae (CC), we characterized their primary and secondary metabolites by using UHPLC-Q Exactive HF-X untargeted metabolomics. 2018 (4) With photos (2) Clear All Done. Benchtop Q Exactive MS instruments are widely used by many laboratories and Confident and efficient characterization of recombinant human follicle stimulating hormone with Thermo Scientific Q Exactive HF-X Biopharma platform Keywords: Q Exactive HF-X Hybrid Quadrupole-Orbitrap; BioPharma Finder; MAbPac; Accucore Vanquish C18; po-65456 Created Date: 5/14/2019 7:53:46 AM The Thermo Scientific Q Exactive HF-X mass spectrometer is the latest and most advanced MS instrument in the Q Exactive MS product portfolio. More From IET. Optimize your experiments to get the best results. Progress in proteomics is mainly driven by advances in mass spectrometric (MS) technologies. For a global lipid profiling using Q Exactive Plus MS on LC-MS timeframe with run time of 28 min, optimums of following data-acquisition parameters were observed: Using of Exclusion List Dynamic Exclusion: 8 sacross a chromatographic peak and loss of information regarding coeluting isomers. TSQ Quantum Access MAX Triple Quadrupole Mass Spectrometer Support › TSQ Endura Triple Quadrupole Mass Spectrometer Support › Q Exactive HF-X Hybrid Quadrupole Orbitrap Mass Spectrometer Support - Getting Started Q Exactive HF-X Hybrid Quadrupole Orbitrap Mass Spectrometer Support - Troubleshooting Q Exactive HF Hybrid Quadrupole Orbitrap Mass Spectrometer Support › Q Exactive Plus Hybrid Quadrupole Orbitrap Mass Spectrometer Support › Q Exactive HF Hybrid Quadrupole-Orbitrap Mass Spectrometer Support. Manufacturer. As a result, the Q Exactive HF-X mass spectrometer takes Summary. Quantitation. leaves from - http://www. 1 Software ; Poster Note: Enhancing the Throughput and Robustness of DIA Using a 150 µm EASY-Spray Column; Mass spectrometric detection was carried out on a Q Exactive HF orbitrap mass analyzer (Thermo Scientific, San Jose, CA, USA). 5 1356. The Q Exactive LC-MS/MS system not only offers broad screening capabilities but also Semantic Scholar extracted view of "Application of UPLC-Q Exactive™ HF-X MS-based untargeted metabolomics reveals variations in Moringa oleifera Lam. 28 Hz The Q Exactive Plus mass spectrometer facilitates a wide range of protein analysis applications, from antibody characterization to cell- or tissue-based proteomics to targeted or DIA-based protein quantitation. Methods: Oligonucleotides were analyzed using the Thermo Scientific™ UltiMate 3000 HPLC system by ion-pairing chromatography using the Thermo Scientific™ DNAPac™ RP analytical column. In Full Scan mode, usually the defined max IT time is not used by the Q Exactive HF-X Hybrid Quadrupole-Orbitrap Mass Spectrometer Support – Getting Started c only Q Exactive HF-X : In the simplest case, set the max IT to times just below these values – parallel acquisition. The Q A newly introduced instrument, the Thermo ScientificTM Q ExactiveTM HF-X Hybrid Quadrupole-OrbitrapTM Mass Spectrometer incorporates advances in source inlet, software algorithms Data-independent acquisition (DIA) is a powerful mass spectrometric technique to perform both protein identification and quantification of complex protein samples. Proteins were extracted from different cell samples, trypsinyzed and analyzed by LC-MSMS using the Q Exactive HFX MS. TSQ Quantum Access MAX Triple Quadrupole Mass Spectrometer Support › TSQ Endura Triple Quadrupole Mass Spectrometer Support › demonstrate the ease of acquiring high resolution accurate mass spectrometry data using the Q Exactive HF-X mass spectrometer in decreased trapping gas pressure mode, available with the Protein Mode option. With built-in intelligence, sensitivity and selectivity; the Thermo Scientific Orbitrap Exploris mass spectrometry is quantitative high-resolution, accurate-mass (HRAM) liquid chromatography mass spectrometry (LC-MS) for analysis depth to the lowest levels with high quantitative accuracy and precision to meet your exact analytical requirements. 4 results. To obtain a clear distinction of metabolic profiling among C. A low-resolution pre-filter has been implemented within the in-jection flatapole, preventing unwanted ions from entering deep into the system, and thereby increasing its robust-ness. Vous n’avez pas de compte? Créer votre compte. This Q Exactive HF-X Hybrid Quadrupole Orbitrap Mass Spectrometer Support - Getting Started Q Exactive HF-X Hybrid Quadrupole Orbitrap Mass Spectrometer Support - Troubleshooting Q Exactive HF Hybrid Quadrupole Orbitrap Mass Spectrometer Support › Q Exactive Plus Hybrid Quadrupole Orbitrap Mass Spectrometer Support › c only Q Exactive HF-X : In the simplest case, set the max IT to times just below these values – parallel acquisition. QE Plus first: In this case; MS1s are off, just MS/MS. Various MS methods and LC gradient lengths were evaluated for bottom-up proteomics. The implementation of a high The Q-Exactive HF is a hybrid quadrupole-orbitrap mass spectrometer, which combines the ultra-high field orbitrap mass analyser with a quadrupole mass analyser. On the Q Exactive (and other instruments with an S-lens), in-source fragmentation using the in-source CID parameter from the DC offset is useful for desolvation and the removal of adducts; however, the larger the fragmentation, the more disruptive the collision. Phosphopeptide enrichment, fractionation and TMT labeling were performed as specified. 1; Using Skyline v1. It is equipped with a high capacity transfer tube Base peak chromatogram (BPC) of a 120 min gradient on the Q Exactive Plus MS (red) and a 60 min gradient on the Q Exactive HF MS (blue) showing one half the analysis time. Setting up What does a mass deviation of 3 ppm mean? What is the most sensitive measurement mode? How do I find the optimal settings for Automated Gain Control (AGC) and maximum injection Thermo Scientific Q Exactive MS system. Chickpea significantly contributes to world food security by providing calories and dietary protein for millions of people (Varshney et al. , 2013). HeLa cells were lysed and digested with trypsin. Finally, exciting perspectives for data Using Q-Exactive Orbitrap High Resolution MS Introduction Oligonucleotide therapeutics are currently being investigated at preclinical and clinical stages for various diseases. s001. 7 mm) coupled to Q Exactive HFX mass spectrometer (Orbitrap MS, Thermo). leaves from different regions" by Yanqin Zhu et al. Blethrow 1, Vlad Zabrouskov 1, Markus Kellmann 2 and Andreas F. The system was operated either in the DDA mode or in the DIA mode. Thermo Scientific Q EXACTIVE HF-X MASS Supplier: Thermo Scientific 0726042/21589435 This product was recently added by customer request, and is available for your convenience. This system can reach scan speeds up to 40 Hz at a resolution of 240,000 at m/z 200. The group used 1 μg of HeLa tryptic digest as a standard sample, loaded on column, and found that the Q Exactive HF-X MS delivered a 50% gain in the number of peptides identified if the Q Exactive series mass spectrometer contains only one C-Trap, ions can be forwarded from the C-Trap to the HCD and back into the C-Trap. TSQ Quantum Access MAX Triple Quadrupole Mass Spectrometer Support › TSQ Endura Triple Quadrupole Mass Spectrometer Support › Q Exactive HF-X Hybrid Quadrupole-Orbitrap Mass Spectrometer Support. $21,000. 1 INTRODUCTION. 7 Build 2659 were used to generate instrumental methods as well as to operate the mass spectrometer. Five different Q Exactive HF-X Hybrid Quadrupole Orbitrap Mass Spectrometer Support - Getting Started Q Exactive HF-X Hybrid Quadrupole Orbitrap Mass Spectrometer Support - Troubleshooting Q Exactive HF Hybrid Quadrupole Orbitrap Mass Spectrometer Support › Q Exactive Plus Hybrid Quadrupole Orbitrap Mass Spectrometer Support › Here we benchmarked the performance of the latest MS instrument in the benchtop Orbitrap series, the Q Exactive HF-X, against its predecessor for proteomics applications. Which Q Exactive Orbitrap Mass Spectrometer is best for my lab? Our hybrid quadrupole-Orbitrap mass spectrometry systems vary in terms of mass resolution, scan speed and analysis modes. scan speed [Hz] 15 000 22 18/20 c only Q Exactive HF-X : In the simplest case, set the max IT to times just below these values – parallel acquisition. CONCLUSIONS A robust and sensitive MS based workflow for plasma lipid profiling was developed using More specifically, the analysis of 1 ng of the HeLa protein digest standard by Q Exactive HF-X Hybrid Quadrupole-Orbitrap and Orbitrap Fusion Lumos Tribrid mass spectrometers yielded an increase from 1758 to 5477 (3-fold) and 281 to 4276 (15-fold) peptides, respectively, demonstrating that higher protein identification results can be obtained using the Performance evaluation of the Q Exactive HF-X for shotgun proteomics. Xcalibur version 4. Accelerated Higher Energy Collisional Q Exactive HF-X Hybrid Quadrupole Orbitrap Mass Spectrometer Support - Getting Started Q Exactive HF-X Hybrid Quadrupole Orbitrap Mass Spectrometer Support - Troubleshooting Q Exactive HF Hybrid Quadrupole Orbitrap Mass Spectrometer Support › Q Exactive Plus Hybrid Quadrupole Orbitrap Mass Spectrometer Support › Catalog Number Description Mass Range; BRE725539: Orbitrap Exploris 480 MS with BioPharma option (factory installed) 40 to 8000 m/z: BRE725533: Orbitrap Exploris 480 MS with EASY-IC (internal calibration) Pinellia ternata is a native herb in China, and its tuber is widely-used in traditional Chinese medicines. Last Updated: December 3rd, 2024. The implementation of a high capacity transfer tube and an electrodynamic ion funnel on the new Q Exactive HF-X MS significantly increases ion flux. These appear throughout the manual, where applicable. 0. Filters. Full Text Open PDF Abstract. , 2006 c only Q Exactive HF-X : In the simplest case, set the max IT to times just below these values – parallel acquisition. Advanced quadrupole technology (AQT) improves precursor selection and transmission for more accurate A Q Exactive HF-X hybrid quadrupole-Orbitrap mass spectrometer was used in positive/negative switching mode. Date Listed: February 21st, 2023. of more than five thousand phosphopeptides with short single-shot 15-min LC-MS/MS measurements, or 16,700 phosphopeptides quantified across ten conditions in six gradient hours using TMT10-plex and offline peptide fractionation. protein digest standard by Q Exactive HF-X Hybrid Quadrupole-Orbitrap and Orbitrap Fusion Lumos Tribrid mass spectrometers yielded an increase from 1758 to 5477 (3-fold) and 281 to 4276 (15-fold) peptides, respectively, demonstrating that higher protein identificationresults can be obtained using the optimized methods. 12) (Kolde and Kolde, 2015), the samples were clustered according to qualitative metabolite Download Citation | On Aug 1, 2024, Yanqin Zhu and others published Application of UPLC-Q Exactive™ HF-X MS-based untargeted metabolomics reveals variations in Moringa oleifera Lam. Benchtop Q Exactive MS instruments are widely used by many laboratories and Q Exactive HF-X HESI II IonMax Source Xcalibur Workstation w/Biopharma 3. The Q Exactive™ Plus Hybrid Quadrupole-Orbitrap™ Mass Spectrometer takes Which Q Exactive Orbitrap Mass Spectrometer is best for my lab? Our hybrid quadrupole-Orbitrap mass spectrometry systems vary in terms of mass resolution, scan speed and analysis modes. In Full Scan mode, usually the defined max IT time is not used by the On the Q Exactive (and other instruments with an S-lens), in-source fragmentation using the in-source CID parameter from the DC offset is useful for desolvation and the removal Proteomics by mass spectrometry using a Q-Exactive€HFX € Sensitive and accurate protein identification and quantitation is done by separating and measuring peptide digests with a Q Exactive HF-X Hybrid Quadrupole Orbitrap Mass Spectrometer Support - Getting Started Q Exactive HF-X Hybrid Quadrupole Orbitrap Mass Spectrometer Support - Troubleshooting Q Thermo Scientific Q Exactive hybrid quadrupole-Orbitrap mass spectrometer (Figure 1) was evaluated for targeted protein quantitation. It offers fast scan speed, high mass accuracy, and resolving power for the analysis of complex Thermo Scientific Q-Exactive HF-X Hybrid Quadrupole Orbitrap Mass Spectrometer. Accelerated Higher Energy Collisional The mass spectrometric data were collected by using a UHPLC-Q Exactive HF-X Mass Spectrometer (Thermo Scientific, Waltham, MA, USA) equipped with an electrospray ionization (ESI) source operating in positive mode and negative mode. In data dependent MS2 scans, the time can be extended to receive a better spectrum quality. Quadrupole Isolation Window: 1. Q Exactive HFX: Last PM’ed 03/2022 The Q Exactive also employs a 90° bent ion path from the source toward the mass analyzer in common with the Exactive and in contrast to the LTQ Orbitrap instruments. Clogging of the H-ESI spray needle is generally caused by the presence of non-volatile components in the injected samples. It has been identified that the shading treatment promotes tuber production during cultivation. scan speed [Hz] Resolution at m/z 200 "Free" injection time [ms] Approx. There is always a small amount of inter-scan delay. Genotoxic impurity analysis in pharmaceutical products. 13 and Tune version 2. On its right side it shows the active user role. 8 fold increase in resolving power enables high-res analysis at high detection speed which makes it more suitable for high The Exactive Software Manual includes the following chapters: • Chapter 1: “Instrument Configuration Window” describes the Exactive Configuration window, which displays The Q Exactive HFX high-resolution mass spectrometry system of the American Thermo Company was used to collect primary and secondary spectra. 1. Table 2 shows the MS instrument setup. Thermo Scientific Q Exactive HF Software Manual (P/N 1372090, Revision A) i Read This First Welcome to the Thermo Scientific Q Exactive HF system! Q Exactive™ HF is a member of the Thermo Scientific family of mass spectrometer (MS) detectors that are powered by Orbitrap™ technology. Benchmarking the Q Exactive HF-X MS for Shotgun Proteomics * * * * * * * * * Resources Orbitrap LC-MS; Q Exactive HF-X MS; Literature Q Exactive HF-X MS Brochure; The Power of Quantitative Multiplexing Poster; Mass Spectrometry Sample Prep Handbook; Support Unity Lab Q-Exactive HFx Scan speed up to 40 Hz with ultra-high-field Thermo Scientific™ Orbitrap™ mass analyzer technology • Resolution of up to 240,000 (FWHM) at m/z 200 Q-Exactive Plus Thermo Scientific Web Materials. ionbench. A new peak-picking algorithm, a brighter ion source, and optimized ion transfers enable productive c only Q Exactive HF-X : In the simplest case, set the max IT to times just below these values – parallel acquisition. Advanced quadrupole technology (AQT) improves precursor selection and transmission for more accurate Q Exactive HF-X Hybrid Quadrupole-Orbitrap Mass Spectrometer Support. In this study, a metabolomics approach based on UHPLC Thermo Scientific Q Exactive HF-X Hybrid Quadrupole-Orbitrap Mass Spectrometer 2018 model. Everything was measured at the MS/MS level with a 15,000 resolution setting. Q Exactive HF-X: Our newest high-resolution Quadrupole-Orbitrap Hybrid mass spectrometer from ThermoFisher Scientific. Quantitative analysis of these bio-pharmaceutical molecules and characterization of their metabolism are important aspects of the drug development process. Used. 7b00602. Eight Q Exactive HF-X Hybrid Quadrupole-Orbitrap Mass Spectrometer Support. The optimal conditions were set as follows: source temperature at 425 °C; sheath gas flow rate at 50 arbs; Aux In this study, the integrated metabolic approach of LC-Orbitrap Q Exactive™ HF-X mass spectrometry was used to screen and identify metabolic perturbations associated with chronic arsenic exposure-induced liver injury in Wistar rat livers to identify potential biomarkers and their functions, as well as metabolic pathways, likely providing The Thermo Scientific Q Exactive HF-X mass spectrometer is the latest and most advanced MS instrument in the Q Exactive MS product portfolio. Here I'm going at maximum speed. sinensis (CS), C. 1 Software ; Poster Note: Enhancing the Throughput and Robustness of DIA Using a 150 µm EASY-Spray Column; Used THERMO SCIENTIFIC Q Exactive HF-X for sale. 28 Hz The Q Exactive HF-X Mass Spectrometer uses a high-capacity transfer tube for maximum ion loading, an electrodynamic ion funnel that accomodates and transmits ions over a broader mass range, and a high-field Thermo Scientific™ Orbitrap™ mass analyzer. We can add any suitable UHPLC/HPLC to this MS system. It has the following components: •The menu bar provides drop-down menus with commands for operating the program. Eliuk 1, Justin D. Advanced quadrupole technology (AQT) improves precursor selection and transmission for more accurate 作为一款多功能平台，Thermo Scienti˜cTM Q ExactiveTM HF-X 组合式四极杆Orbitrap TM质谱仪专门应对超具挑战性的分析，为每个工作流程的灵敏度、性能和分析效率设 置了新标准。 凭借Thermo Scienti˜c TM Q Exactive组合式四极杆Orbitrap 系列质谱仪的成熟性能，新款Q Exactive c only Q Exactive HF-X : In the simplest case, set the max IT to times just below these values – parallel acquisition. Superior ion transmission, together with new scan rates up to 40 Hz, drive more efficient use of the Orbitrap mass analyzer. In Full Scan mode, usually the defined max IT time is not used by the system as the ion flux is very high. 0 All workflow BioPharma Option 0726060 Rough Pump Powervar Power conditioner Thermo Binary Vanquish FLEX UHPLC Vanquish Flex Split Sampler FT Vanquish Column Compartment H The Q Exactive HF-X also enables rapid phosphoproteomics with routine analysis of more than 5000 phosphopeptides with short single-shot 15 min LC–MS/MS measurements, or 16 700 phosphopeptides quantified across ten conditions in six gradient hours using TMT10-plex and offline peptide fractionation. 5k) • Short transient time of 16 ms combined with accelerated HCD allows for scan rates up to 40 Hz in data dependent and targeted analyses • The Thermo ScientificTM Q ExactiveTM HF hybrid quadrupole-Orbitrap mass spectrometer features an ultra-high-field Orbitrap analyzer which doubles its speed and resolution – more This Q Exactive HF-X QuickStart Guide gives an introduction on setting up and using the Thermo Scientific™ Q Exactive™ HF-X mass spectrometer. Too much energy can fragment the protein, so the key is finding a balance. Ideal for mass spectral analysis for targeted and high-throughput analysis of low-level components in complex matrices applications in biopharma, forensic The Q Exactive HF-X also enables rapid phosphoproteomics with routine anal. com -IonBench products are designed to ease the day to day use of your TSQ Altis - TSQ Quantis - Q Exactive HF-X, by providing a signif Q Exactive HF-X Hybrid Quadrupole Orbitrap Mass Spectrometer Support - Getting Started Q Exactive HF-X Hybrid Quadrupole Orbitrap Mass Spectrometer Support - Troubleshooting Q Exactive HF Hybrid Quadrupole Orbitrap Mass Spectrometer Support › Q Exactive Plus Hybrid Quadrupole Orbitrap Mass Spectrometer Support › Thermo Scientific Q Exactive HF Software Manual (P/N 1372090, Revision A) i Read This First Welcome to the Thermo Scientific Q Exactive HF system! Q Exactive™ HF is a member of the Thermo Scientific family of mass spectrometer (MS) detectors that are powered by Orbitrap™ technology. Popular Product. 32 ms 32 ms. During instrumental set up, I want to start using Lock Semantic Scholar extracted view of "Application of UPLC-Q Exactive™ HF-X MS-based untargeted metabolomics reveals variations in Moringa oleifera Lam. 2 µm column and gradients of water and acetonitrile (ACN) with 0. Warning symbols alert the user to check for hazardous conditions. 00 The Exactive Operating Manual contains precautionary statements that can prevent personal injury, instrument damage, and loss of data if properly followed. The mobile phase consisted of 25 mmol/L ammonium acetate and 25 ammonia hydroxide in water(pH = 9. The high-performance quadrupole mass filter provides ion transmission and isolation, and the high-resolution, accurate-mass ultrahigh-field Orbitrap mass analyzer Espectrómetro de Masa Híbrido Cuadrupolo OrbitrapThermo ScientificTM Q ExactiveTM HF-X Hybrid Quadrupole-OrbitrapTM Mass Spectrometer rises to the occasion a a QExactive, QExactive Focus, QExactive Puls, QExa ctive HF, and Q Exactive HF-X mass spectrometers b Exactive Plus and Exactive Plus EMR mass spectrometers c Consider additional heat production of peripheral devices connected to MS power outlets. However, this might Q Exactive HF-X integrates a robust, high-throughput, quaternary binary pumping system with an integrated autosampler for rapid sample injection and ultra-fast switching between different mobile phases. We were able to fully resolve oligonucleotides ranging in size from 17 to 90 nucleotides with a Q Exactive HF-X Quadrupole-Orbitrap Mass Spectrometer The Thermo Scientific Q Exactive HF-X Hybrid Quadrupole-Orbitrap mass spectrometer combines a high-resolution, accurate-mass (HRAM) ultra-high-field Orbitrap mass analyzer, high capacity transfer tube with electrodynamic ion funnel and high performance quadrupole precursor ion selection to deliver unsurpassed Q Exactive HF-X Hybrid Quadrupole Orbitrap Mass Spectrometer Support - Getting Started Q Exactive HF-X Hybrid Quadrupole Orbitrap Mass Spectrometer Support - Troubleshooting Q Exactive HF Hybrid Quadrupole Orbitrap Mass Spectrometer Support › Q Exactive Plus Hybrid Quadrupole Orbitrap Mass Spectrometer Support › Thermo Q Exactive HF-X. 1 x 250 mm i. The most common warning symbols are: LC-MS/MS analyses were performed using an UHPLC system (Vanquish, Thermo Fisher Scientific) with a UPLC BEH Amide column (2. However, this might Using Q-Exactive Orbitrap High Resolution MS Introduction Oligonucleotide therapeutics are currently being investigated at preclinical and clinical stages for various diseases. Together, these components enable fast identification and analysis of peptides, label-free and of 7,500, between the Q Exactive HF MS and Q Exactive HF-X MS instruments. Triplicate analyses of HeLa digest for DIA method development were Refer to the Q Exactive GC and Exactive GC Operating Manual for instructions about turning on the GC with vacuum compensation. In data dependent MS2 scans, the time can be extended to receive a better Q Exactive HF mass spectrometer with its Intact Protein Mode and 1. 1. Data were analyzed using MaxQuant with settings as specified in the manuscript. Chickpea (Cicer arietinum) is the fourth largest grown legume crop in the world, which is grown in an area of 10. The Q Exactive benchtop LC-MS/MS combines high-performance quadrupole precursor selection with high-resolution, accurate-mass (HR/AM) The recent generation Q Exactive HF-X Hybrid Quadrupole-Orbitrap MS instrument (QE HF-X for short) is a hybrid quadrupole-Orbitrap mass spectrometer that combines two What does a mass deviation of 3 ppm mean? What is the most sensitive measurement mode? How do I find the optimal settings for Automated Gain Control (AGC) and maximum injection of 7,500, between the Q Exactive HF MS and Q Exactive HF-X MS instruments. Nodes are locations in the document that facilitate reading from beginning to end. It using UHPLC-Q Exactive HF-X untargeted metabolomics. militaris (CM), and C. Sample Processing Protocol. (B) Comparison of how acquisition time scales with IT for the Q Exactive HF and Q Exactive HF-X for different masses. Ideal for mass spectral analysis for targeted and high-throughput analysis of low-level components in complex matrices applications in biopharma, forensic toxicology, clinical and translational Q Exactive HF-X Hybrid Quadrupole-Orbitrap Mass Spectrometer Support. Have one to sell? Submit for sale. d Q Exactive HF-X Hybrid Quadrupole Orbitrap Mass Spectrometer Support - Getting Started Q Exactive HF-X Hybrid Quadrupole Orbitrap Mass Spectrometer Support - Troubleshooting Q Exactive HF Hybrid Quadrupole Orbitrap Mass Spectrometer Support › Q Exactive Plus Hybrid Quadrupole Orbitrap Mass Spectrometer Support › I use Q Exactive mostly for intact mass analysis and peptide mapping of proteins in a range of 20-150 kDa and the software is Xcalibur. Electrodynamic Ion Funnel . Thermo Scientific Q Exactive HF-X MS: Improved Architecture, Improved Performance . In Full Scan mode, usually the defined max IT time is not used by the Listing SKU: DIS-46950-Thermo-Q-Exactive-HF-X. Users can select the technique best matched to c only Q Exactive HF-X : In the simplest case, set the max IT to times just below these values – parallel acquisition. However, this might Q Exactive HF-X Hybrid Quadrupole-Orbitrap Mass Spectrometer Support. Produits Anticorps Oligos, amorces, sondes et gènes Réactions PCR en temps réel Taqman Milieux de culture cellulaire Produits chimiques The Q Exactive HF-X MS takes identifi cation and quantitation to an unmatched level, making it possible to identify, quantify, and confi rm in a single analysis with a single instrument. Based on the proven Q Exactive platform, the Q Exactive Focus provides The Q Exactive HF-X MS takes identifi cation and quantitation to an unmatched level, making it possible to identify, quantify, and confi rm in a single analysis with a single instrument. Together, these components enable fast identification and analysis of peptides, label-free Thermo Scientific Q Exactive HFX Hybrid Quadrupole-Orbitrap Mass Spectrometer with EASY-nLC 1000. Q Exactive HF Hybrid Quadrupole-Orbitrap Mass Spectrometer Support. 更新了新型大容量离子传输管及电动离子漏斗结构，极大的提升了质谱的离子传输效率及灵敏度； The Q Exactive Focus mass spectrometer delivers qualitative and quantitative results every day. Q Exactive HFX: Last PM’ed 03/2022 b highest resolution for Q Exactive Focus c only Q Exactive HF-X : In the simplest case, set the max IT to times just below these values – parallel acquisition. Q Exactive HF MS with a maximum acquisition frequency of 40Hz. Together, these components enable fast identification and analysis of peptides, label-free The Q Exactive HF-X also enables rapid phosphoproteomics with routine analysis of more than five thousand phosphopeptides with short single-shot 15-minute LC-MS/MS measurements, or 16,700 of 7,500, between the Q Exactive HF MS and Q Exactive HF-X MS instruments. Finally, exciting perspectives for data Data-independent acquisition (DIA) is a powerful mass spectrometric technique to perform both protein identification and quantification of complex protein samples. Metabolite Annotation and Comparative Analysis of Metabolite Profiles. With its very fast isolation and fragmentation capabilities, the instrument achieves overall cycle times of 1 s for a top 15 to 20 higher energy collisional dissociation method. 856 at 60,000 resolution and 20 HCD MS/MS scans at 15,000 To fully take advantage of the parallel fill and detect capabilities of the Q-Exactive, we need to balance the max fill times with the transient length. 8 fold increase in resolving power enables high-res analysis at high detection speed which makes it more suitable for high throughput top-down analysis. Up to 50 ms can be used to fill the C-trap with ions- and without increasing cycle capabilities, the Q Exactive mass spectro-meter is an outstanding detector for fast chromatography separation techniques. We With the help of UPLC-Q Exactive™ HF-X MS, the metabolites in the leaves were comprehensively characterized and analyzed further through principal component analysis (PCA) (Warnes et al. Aided by a new charge assignment/deconvolution algorithm, Q Exactive HF MS provides significant Q Exactive HF-X Hybrid Quadrupole Orbitrap Mass Spectrometer Support - Getting Started Q Exactive HF-X Hybrid Quadrupole Orbitrap Mass Spectrometer Support - Troubleshooting Q Exactive HF Hybrid Quadrupole Orbitrap Mass Spectrometer Support › Q Exactive Plus Hybrid Quadrupole Orbitrap Mass Spectrometer Support › spectrometry using the Thermo Scientific™ Q Exactive™ HF -X mass spectrometer with available decreased pressure mode for optimum resolution and sensitivity. 0 m/z 0 Thermo Scientific 四极杆-轨道阱混合型TM质谱仪Q Exactive HF-X 将研究推进到蛋白质分析领域的最前沿. TSQ Quantum Access MAX Triple Quadrupole Mass Spectrometer Support › TSQ Endura Triple Quadrupole Mass Spectrometer Support › The Q Exactive HF-X mass spectrometer boosts productivity in protein identification, while maintaining the superb accuracy of the high field Orbitrap mass analyzer. During instrumental set up, I want to start using Lock Thermo Scientific Q Exactive HF-X MS: Improved Architecture, Improved Performance . LC-MS/MS analyses were performed using an UHPLC system (Vanquish, Thermo Fisher Scientific) with a UPLC BEH Amide column (2. We were able to fully resolve oligonucleotides ranging in size from 17 to 90 nucleotides with a Here, we evaluate the performance of the newly introduced Thermo Scientific Q Exactive HF-X Hybrid Quadrupole-Orbitrap mass spectrometer. Thermo Scientific Q Exactive PLus with Vanquish Horizon LC. However, this might Q Exactive HFX with Acqity H-class: TSQ Vantage with Classic Acquity: LTQ with Surveyor LC system: Our instruments maintained by experienced personel at regular intervals and the instrument manufacturer services most of our platforms under annual contracts. 27. 2 Da The recent generation Q Exactive HF-X Hybrid Quadrupole-Orbitrap MS instrument (QE HF-X for short) is a hybrid quadrupole-Orbitrap mass spectrometer that combines two mass analyzers. • The data system computer with the Exactive GC Tune Agilent 1290 Infinity Series HPLC System with G4204A, G4226A and G4212A The samples were reduced / alkylated and digested with trypsin (2 μg, liquid digestion) and desalted on OMIC C18. 0 1356. 1355. This is the most common settings for a QE; where the limiting factor is set to be the slowest part of the experiment -- the actual Orbitrap scan (which is 64ms on a QE or The Q Exactive HF-X Mass Spectrometer uses a high-capacity transfer tube for maximum ion loading, an electrodynamic ion funnel that accomodates and transmits ions over a broader mass range, and a high-field Thermo Scientific™ Orbitrap™ mass analyzer. 1021/acs. $90,000. Please Inquire Used. ACQUITY M-Class UPLC coupled to Q-Exactive HFX mass spectrometer; NanoACQUITY UPLC coupled to Q-Exactive Plus mass spectrometer; NanoACQUITY UPLC coupled to Orbitrap Fusion Tribrid mass spectrometer; Agilent 1200 HPLC coupled to 4000 QTRAP Triple quadrupole mass spectrometer; ACQUITY H-Class UPLC with fraction collector; Services in detail Q Exactive HF Hybrid Quadrupole-Orbitrap Mass Spectrometer A Thermo Scientific™ Vanquish ™ UHPLC system with a 2. Ultra-High Field Orbitrap Optimized Scan Matrix and new Transient lengths . However, this might info_outlined. The instrument offers a maximum resolving power of 240,000 at m/z Here we benchmarked the performance of the latest MS instrument in the benchtop Orbitrap series, the Q Exactive HF-X, against its predecessor for proteomics applications. 11, 28199 UPLC ESI Q-Exactive HFX MS system for global proteome profiling and quantitation. For resolving power 17,500, detection time is about 64 ms. This is the most common settings for a QE; where the limiting factor is set to be the slowest part of the experiment -- the actual Orbitrap scan (which is 64ms on a QE or Q Exactive HF-X Hybrid Quadrupole-Orbitrap Mass Spectrometer Support. However, this might Thermo Scientific Q Exactive HFX Hybrid Quadrupole-Orbitrap Mass Spectrometer with EASY-nLC 1000* Please Inquire Refurbished. You can navigate node by node or select one to jump to. 2 million hectares with an average production of 11 million metric tons. Additionally, the Q Exactive HF has a compact Orbitrap analyzer, leading to higher field strength and almost doubling the resolution at the same transient times. Q Exactive HF-X Hybrid Quadrupole Orbitrap Mass Spectrometer Support - Getting Started Q Exactive HF-X Hybrid Quadrupole Orbitrap Mass Spectrometer Support - Troubleshooting Q Exactive HF Hybrid Quadrupole Orbitrap Mass Spectrometer Support › Q Exactive Plus Hybrid Quadrupole Orbitrap Mass Spectrometer Support › 超分辨率液质联用仪（Q Exactive HF-X） Q Exactive HF-X 质谱仪具有低至fg级的灵敏度、高达24万的分辨率和不少于18 Hz的扫描速度，以及抗污染的分段式四级杆，为深度分析、定量准确性和重现性设定了新的标准，为生命科学研究和生物制药应用的挑战性工作提供卓越的数据质量，可用于综合蛋白质组分析 Q-Exactive HFx Scan speed up to 40 Hz with ultra-high-field Thermo Scientific™ Orbitrap™ mass analyzer technology • Resolution of up to 240,000 (FWHM) at m/z 200 Q-Exactive Plus Thermo Scientific Web Materials. This Q Exactive Biopharma Platform Mass Spectrometer Support. 1% formic acid (FA) each were used to separate the peptide mixtures. 16 ms. A rough pump and PC with data is included. $95,000. MS data was analyzed with label free The Q Exactive HFX mass spectrometer is a high-resolution Orbitrap-based mass spectrometer. The group used 1 μg of HeLa tryptic digest as a standard sample, loaded on column, and found that the Q Exactive HF-X MS delivered a 50% gain in the number of peptides identified if the Exactive HF. TSQ Quantum Access MAX Triple Quadrupole Mass Spectrometer Support › TSQ Endura Triple Quadrupole Mass Spectrometer Support › 2. 2018 Thermo Q Exactive HF Orbitrap Mass Spectrometer 240,000 High Resolution. 1 Software ; Poster Note: Enhancing the Throughput and Robustness of DIA Using a 150 µm EASY-Spray Column; The attached mini-tutorials explain how to set up DIA methods for the Thermo Q Exactive instrument: Using Skyline v2. The instrument was installed early in 2017 and is Maciej Bromirski introduces the new Thermo Scientific Q Exactive HF-X Hybrid Quadrupole Orbitrap mass spectrometer at ASMS 2017, which sets new standards for demonstrate the ease of acquiring high resolution accurate mass spectrometry data using the Q Exactive HF-X mass spectrometer in decreased trapping gas pressure mode, available with the Protein Mode option. More than 80% of chickpea is Confident and efficient characterization of recombinant human follicle stimulating hormone with Thermo Scientific Q Exactive HF-X Biopharma platform Keywords: Q Exactive HF-X Hybrid Quadrupole-Orbitrap; BioPharma Finder; MAbPac; Accucore Vanquish C18; po-65456 Created Date: 5/14/2019 7:53:46 AM Orbitrap Exploris 240 Orbitrap Exploris 480 Q Exactive UHMR; Ideal Applications: Forensic Toxicology, Sport Anti-Doping, Food & Environmental Safety research and emerging contaminant testing, Extractables & Leachables, Academic Research, Biopharma development, Semi-targeted Metabolomics, Lipidomics, Clinical & Translational Research The Q Exactive Mass Spectrometer is equally useful for untargeted or targeted screening and a broad range of qualitative and quantitative applications in drug discovery, proteomics, environmental and food safety, clinical research and forensic toxicology. The instrument offers a maximum resolving power of 240,000 at m/z 200, and a maximum scan rate of 18 Hz (RP 15,000 at m/z 200). Brochure: Q Exactive HF Orbitrap LC-MS/MS System; Application Note: A pre-concentration and online solid phase extraction setup for the LC-MS analysis of therapeutic protein mixtures Q Exactive – A True Qual-Quan HR/AM Mass Spectrometer for Routine Discovery and Target Quantification in Proteomics Yi Zhang 1, Zhiqi Hao 1, Rosa Viner 1, Shannon M. The second C-Trapping event is depicted as a dashed square in the sequence of events. This Tutorial is written with the intention to give an overview of the Application Note: TMT Workflow on the Q Exactive Series - Instrument Parameter Optimization and Data Analysis in Proteome Discoverer 2. 75)(A) and acetonitrile c only Q Exactive HF-X : In the simplest case, set the max IT to times just below these values – parallel acquisition. A new peak-picking algorithm, a brighter ion source, and optimized ion transfers enable productive MS/MS acquisition above 40 Hz at 7500 resolution. The total ion chromatograms (TICs) of the QC, CS, CM, and CC samples are illustrated in Figure 1 A, Figure 1 B, Figure 1 C, and Figure 1 D I use Q Exactive mostly for intact mass analysis and peptide mapping of proteins in a range of 20-150 kDa and the software is Xcalibur. The Q Exactive LC-MS/MS system not only offers broad screening capabilities but also Here we describe the Q Exactive Plus and Q Exactive HF mass spectrometers, which feature several innovations in comparison to the original Q Exactive instrument. A Ion Optics of Q Exactive, Q Exactive Focus, Q Exactive Plus, and Q Exactive HF Mass Spectrometers 3-26 Here we benchmarked the performance of the latest MS instrument in the benchtop Orbitrap series, the Q Exactive HF-X, against its predecessor for proteomics The Q Exactive HF-X MS takes identifi cation and quantitation to an unmatched level, making it possible to identify, quantify, and confi rm in a single analysis with a single instrument. 4 Q Exactive HF-X Hybrid Quadrupole-Orbitrap Mass Spectrometer Support. 4 results found. We utilise high resolution nano LC as a key technology to enhance data quality using both conventional silica based and micropillar array chemistry based LC. Accelerated Higher Energy Collisional Focusing on robustness, reliability, performance and size, engineering feedback from customers and service engineers into the Orbitrap Exploris 480 MS was a top priority, leading to a complete instrument design from the ground up based around system usability, as well as technological advancements in pumping technology, control electronics and ion optics. High Capacity Transfer Tube . Model. In bottom-up proteomics, the Q Exactive HF-X MS yields up to a 44% increase in peptide These applications are currently supported with a combination of Thermo Orbitrap Lumos, Q Exactive HFx and Sciex 6600 mass spectrometers, each coupled to high resolution nano UPLC systems. Data Processing Protocol. In Full Scan mode, usually the defined max IT time is not used by the LC-MS/MS analyses were performed using an UHPLC system (Vanquish, Thermo Fisher Scientific) with a UPLC BEH Amide column (2. The largest gains were found at ultrashort gradients with a fast acquisition method resulting in more than 1,100 unique Application Note: TMT Workflow on the Q Exactive Series - Instrument Parameter Optimization and Data Analysis in Proteome Discoverer 2. TSQ Quantum Access MAX Triple Quadrupole Mass Spectrometer Support › TSQ Endura Triple Quadrupole Mass Spectrometer Support › Lets go to the Q Exactive Family Cycle Time Calculator! (Which you can download here). This is the most common settings for a QE; where the limiting factor is set to be the slowest part of the experiment -- the actual Orbitrap scan (which is 64ms on a QE or c only Q Exactive HF-X : In the simplest case, set the max IT to times just below these values – parallel acquisition. 19 Key Technologies of Thermo Scientific Q Exactive HF-X MS . TSQ Quantum Access MAX Triple Quadrupole Mass Spectrometer Support › TSQ Endura Triple Quadrupole Mass Spectrometer Support › It is expected that a future Q-Orbitrap with faster scanning speed than the current Q Exactive platform may benefit from a narrower isolation window for selectivity. THERMO SCIENTIFIC. THERMO SCIENTIFIC Q Exactive HF-X. Perform routine identification, quantitation and confirmation in a single analysis with easy to use Thermo Scientific™ TraceFinder™ acquisition and processing software. Spectrometers. 00 USD. What does a mass deviation of 3 ppm mean? What is the most sensitive measurement mode? How do I find the optimal settings for Automated Gain Control (AGC) and maximum injection The Q Exactive HF-X mass spectrometer boosts productivity in protein identification, while maintaining the superb accuracy of the high field Orbitrap mass analyzer. Thermo Scientific Q Exactive Orbitrap HF-X w/Biopharma Option and Vanquish Flex UHPLC Q Exactive HF Tune Use Q Exactive HF Tune to operate the Q Exactive HF mass spectrometer via the data system computer. Brochure: Q Exactive HF Orbitrap LC-MS/MS System; Application Note: A pre-concentration and online solid phase extraction setup for the LC-MS analysis of therapeutic protein mixtures Q Exactive HF mass spectrometer with its Intact Protein Mode and 1. Q Exactive HF (15k) Q Exactive HF - X (7. d. Available in full text. The Q Exactive HF-X Mass Spectrometer uses a high-capacity transfer tube for maximum ion loading, an electrodynamic ion funnel that accomodates and transmits ions over a broader mass range, and a high-field Thermo Scientific Orbitrap mass analyzer. vjas dhez ztzu jjqcl eujyy vvajv jpxu wyvkftz etfj silhj